TUBB antibody (N-Term) (Alexa Fluor 555)

Catalog No. ABIN305890

This Rabbit Monoclonal antibody specifically detects TUBB in IHC, IF, ICC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for TUBB antibody (N-Term) (Alexa Fluor 555) (ABIN305890)

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TUBB antibody is conjugated to Alexa Fluor 555

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 9F3

Product Details anti-TUBB Antibody (Alexa Fluor 555)

Binding Specificity: N-Term

Specificity: Detects endogenous levels of total human beta-Tubulin protein.

Purification: Purified

Immunogen: Synthetic phosphopeptide (KLH-coupled) corresponding to the amino terminus of human beta-Tubulin.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IF, IHC, IHC-P (1:100)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, less then 0.1 % sodium azide, 2 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Product should be stored undiluted, is photosensitive and should be protected from light.

Storage: 4 °C

Storage Comment: Store at 4°C undiluted. Do not freeze. Protect from light.

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: TUBB / Beta Tubulin

Background: Name/Gene ID: TUBB

Synonyms: TUBB, Beta Tubulin, Beta 5-tubulin, Beta-4 tubulin, Beta1-tubulin, M40, Tubulin beta-5 chain, Beta Ib tubulin, Class I beta-tubulin, OK/SW-cl.56, TUBB5, Tubulin beta chain, Tubulin beta polypeptide, Tubulin beta-1 chain, Tubulin, beta class I, Tubulin, beta polypeptide

Gene ID: 203068

UniProt: P07437

Pathways: Microtubule Dynamics, M Phase