IGF1R antibody (PE)

Catalog No. ABIN306224

The Mouse Monoclonal anti-IGF1R antibody is suitable to detect IGF1R in samples from Human. It has been validated for WB, IHC, FACS and IHC (p).

Quick Overview for IGF1R antibody (PE) (ABIN306224)

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGF1R antibody is conjugated to PE

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IGF1R Antibody (PE)

Specificity: Recognizes human CD221, a 155kD receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. Recognizes an epitope in the alpha subunits of CD221, demonstrated by Western blotting. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins. Clone 1H7 is reported to partially block binding of IGF-I and IGF-II to CD221

Purification: Protein G purified

Immunogen:

Type of Immunogen: Native protein

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:1), IHC, IHC-P, WB

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 1 mL sterile distilled water

Concentration: Lot specific

Buffer: Lyophilized, PBS, 0.09 % sodium azide, 1 % BSA, 5 % sucrose

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: IGF1R / IGF1 Receptor

Background: Name/Gene ID: IGF1R
Subfamily: Insulin receptor
Family: Protein Kinase

Synonyms: IGF1R, CD221 antigen, CD221, IGF1 Receptor, IGFR, IGF-I receptor, Soluble IGF1R variant 1, Soluble IGF1R variant 2, Type 1 IGF receptor, IGFIR, JTK13

Gene ID: 3480

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy