Cytochrome C antibody (full length)

Catalog No. ABIN306559

This Sheep Polyclonal antibody specifically detects Cytochrome C in WB, IHC and IP. It exhibits reactivity toward Human, Rat, Dog and Rabbit.

Quick Overview for Cytochrome C antibody (full length) (ABIN306559)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity: Human, Rat, Dog, Rabbit

Host: Sheep

Clonality: Polyclonal

Conjugate: This Cytochrome C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-Cytochrome C Antibody

Binding Specificity: full length

Purpose: Sheep anti Human Cytochrome c

Cross-Reactivity: Human

Characteristics: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.

Purification: Ammonium Sulfate Precipitation

Immunogen: Full length recombinant Cytochrome C

Isotype: IgG

Application Details

Application Notes: Applications: Western Blot 1:5,000, shows a MW of 14.4 kDa. May be used in immunofluorescence applications with human and rat cells, Immunoprecipitation - Empirical.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: Cytochrome c

Background: Sheep anti cytochrome c, cyto c, cytochrome-c

UniProt: P99999, P62898, P00008, P00011

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity