CDKN2D antibody

Catalog No. ABIN306573

The Rabbit Polyclonal anti-CDKN2D antibody (Clone DCS-100) (ABIN306573) specifically detects CDKN2D in WB, ELISA, IHC (p), IF and RIA. The antibody is reactive with Human samples.

Quick Overview for CDKN2D antibody (ABIN306573)

Target: CDKN2D (Cyclin-Dependent Kinase Inhibitor 2D (p19, Inhibits CDK4) (CDKN2D))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDKN2D antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)

Clone: DCS-100

Product Details anti-CDKN2D Antibody

Cross-Reactivity: Pig

Cross-Reactivity (Details): Pig, cross-reactivity with human < 0.1 % , mouse and rat collagen IV <1 %

Characteristics: hp19, p19 ink4d, CDKN2D, Cyclin-dependent kinase 4 inhibitor D,Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Porcine collagen type IV 100 % , porcine collagens type I, II, and III <0.1 %, porcine elastin, fibronectin and laminin < 0.1 % (RIA at 1:5000 dilution).

Immunogen: Rabbits were immunized with purified collagen type IV from porcine kidney tissue

Isotype: IgG1

Application Details

Application Notes: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,IFA 1:80, IHC(P) 1:500, ELISA >1:200

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Affinity purified antibody lyophilized from phosphate buffered solution, no BSA and preservative added! Reconstitute with 0.1 mL sterile bidistilled water.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: -20°C

Target Details for CDKN2D

Target: CDKN2D (Cyclin-Dependent Kinase Inhibitor 2D (p19, Inhibits CDK4) (CDKN2D))

Alternative Name: p19 - ink4d

Pathways: Cell Division Cycle, Sensory Perception of Sound, Mitotic G1-G1/S Phases, Negative Regulation of intrinsic apoptotic Signaling