SH3BP4 antibody

Catalog No. ABIN306590

The Sheep Polyclonal anti-SH3BP4 antibody is suitable to detect SH3BP4 in samples from Human. It has been validated for WB.

Quick Overview for SH3BP4 antibody (ABIN306590)

Target: SH3BP4 (SH3-Domain Binding Protein 4 (SH3BP4))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This SH3BP4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SH3BP4 Antibody

Purpose: Sheep anti Human Guanalyate Kinase GKWW domain (MAGI)

Cross-Reactivity: Human

Characteristics: Membrane-associated guanylate kinase with inverted orientation (MAGI3) is a novel, 130 kDa guanylate kinase which is part of a family of multi-PDZ domain containing guanylate kinases. These guanylate kinases are localized to epithelial cell tight junctions. MAGI3 and PTEN/MMAC cooperate to modulate the kinase activity of AKT/protein kinase B (PKB). MAGI3 positions the PTEN/MMAC phosphatase to specific subcellular locations that are involved with the regulation of cell proliferation and survival. MAGI3, as well as other proteins in this family, are made up of an N-terminal guanylate kinase domain, followed by a WW domain, named for two conserved tryptophan residues and first identified as two repeats in mouse YAP65, which is then followed by 6 PDZ domains.

Purification: Ammonium Sulfate Precipitation

Immunogen: Recombinant protein corresponding to the guanylate kinase-WW domain of MAGI-3.

Isotype: IgG

Application Details

Application Notes: Antibody detects MAGI-3 by Western blot at 1-10 μg/mL. Detects a 130 kDa band in cells transfected with full-length MAGI-3 protein. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for SH3BP4

Target: SH3BP4 (SH3-Domain Binding Protein 4 (SH3BP4))

Alternative Name: Membrane AssociatedGuanalyate Kinase GKWW domain (MAGI)

Background: Sheep anti-Membrane-associated guanylate cyclase of inverted orientation-3, Guanylate Kinase-WW domains

UniProt: Q9H2V6