PPP2R5D antibody

Catalog No. ABIN306653

The Rabbit Polyclonal anti-PPP2R5D antibody is suitable to detect PPP2R5D in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for PPP2R5D antibody (ABIN306653)

Target: PPP2R5D (Protein Phosphatase 2, Regulatory Subunit B', delta (PPP2R5D))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP2R5D antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PPP2R5D Antibody

Purpose: Rabbit anti Human Protein Phosphatase 2 A/B delta

Cross-Reactivity: Cow, Human, Mouse, Rat

Characteristics: PP2A is a heterotrimeric phosphatase, ccomposed of A, B and C subunits. The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. Three families of B subunits have been identified thus far. The prototypical B (or PR55) family of subunits is encoded by three genes, B , B and B . The B' (or B56) family consists of at least seven splice variants derived from five genes. RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals. B is a novel isoform of the B class of PP2A regulatory subunits. It is widely expressed in rat tissues and appears to be involved in targeting PP2A to the cytosolic compartment.

Purification: Ammonium Sulfate Precipitation

Immunogen: Synthetic peptide conjugated to KLH derived from the N-terminus of PP2A/B delta

Isotype: IgG

Application Details

Application Notes: Detects PP2A/B delta protein in rat testis by Western blot. Detects a 53 kDa band in rat brain, heart, lung, placenta, skeletal muscle, spleen, testis and thymus tissues lysates. Optimal concentration should be evaluated by serial dilutions. Applications: Western Blotting, Immunofluorescence

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for PPP2R5D

Target: PPP2R5D (Protein Phosphatase 2, Regulatory Subunit B', delta (PPP2R5D))

Alternative Name: Protein Phosphatase 2 A/B delta

Background: Pp2A/Bd, rabbit anti protein phosphatase 2A, B subunit delta, PP2A B subunit delta

UniProt: Q14738, Q925E7, P56932

Pathways: PI3K-Akt Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades