Caspase 6 antibody

Catalog No. ABIN306793

This Mouse Monoclonal antibody specifically detects Caspase 6 in WB. It exhibits reactivity toward Human.

Quick Overview for Caspase 6 antibody (ABIN306793)

Target: Caspase 6 (CASP6) (Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 6 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: MCH2 14 1-190

Product Details anti-Caspase 6 Antibody

Purpose: Mouse anti Human Caspase-6

Cross-Reactivity: Human

Characteristics: Caspase-6 (Cysteine-Requiring Aspartate Proteases) is part of a family of intracellular cysteine proteases that cleave their substrates after aspartic acid residues. These proteases play an integral role in inducing apoptosis in cells. Procaspase-6 (Mch2), a member of the ICE/ced-3 subfamily, is an inactive proenzyme that is activated to form caspase-6 by proteolytic cleavage at certain aspartic acid residues. During cleavage, the N-terminal is removed and the proenzyme is converted into a large (p18) and small (p11) subunits. Caspase-6 has two isoforms, and , produced by alternative splicing. Over-expression of the isoform of caspase-6 without its prodomain can induce apoptosis. The isoform does not seem to display proteolytic activity. Together with caspases-3 and -7, the isoform of caspase-6 is classified as an effector/execution caspase. Caspase-3, caspase-8 and caspase-10 can cleave procaspase-6. Active caspase-6 cleaves several other proteins such as lamins, NuMa and Keratin 18. A possible cleavage of caspases-8 and -10 in cytochrome-C dependent apoptosis was reported recently.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human capase-6 protein and mouse myeloma cells.

Isotype: IgG

Application Details

Application Notes: Detects proenzyme form of human Caspase-6 by Western blot. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Caspase 6

Target: Caspase 6 (CASP6) (Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6))

Alternative Name: Caspase-6

Background: Cysteine-requiring Aspartate Protease-6, Apoptotic protease Mch-2

UniProt: P55212

Pathways: Apoptosis, Caspase Cascade in Apoptosis