His Tag antibody

Catalog No. ABIN306800

The Mouse Monoclonal anti-His Tag antibody is suitable to detect His Tag. It has been validated for WB.

Quick Overview for His Tag antibody (ABIN306800)

Target: His Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 2C5-1

Product Details anti-His Tag Antibody

Purpose: Mouse anti His Tag

Sequence: HHHHHH

Characteristics: 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems. This antibody has been found to be effective in detecting proteins expressed using the following vector systems, pET 28, 30 and 32 as well as Invitrogen's pIZ and is specific for 6x-His tags only.

Purification: Protein A/G Chromatography

Isotype: IgG2a

Application Details

Application Notes: Detects only 6x-His tag by Western blot. Specific for His tags no longer than 6 histidines. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for His Tag

Target: His Tag

Target Type: Tag

Background: Anti-6x-Histidine Tag Antibody