RIT1 antibody

Catalog No. ABIN306804

This anti-RIT1 antibody is a Mouse Monoclonal antibody detecting RIT1 in WB. Suitable for Human.

Quick Overview for RIT1 antibody (ABIN306804)

Target: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RIT1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 14G7

Product Details anti-RIT1 Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64 % amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100 % conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.

Purification: Protein A or G purified

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant full-length human Rit protein and mouse myeloma cells.

Isotype: IgG1

Application Details

Application Notes: Antibody detects recombinant Rit protein expressed in 293 cells by Western blot analysis at a concentration of 0.5 to 1 μg/mL. Detects a band of approximately 25 kDa (predicted molecular weight: 26 kDa). Does not cross-react with Rin protein. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50 % glycerol at pH 7.5 and 3 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for RIT1

Target: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Alternative Name: Rit

UniProt: Q92963

Pathways: Neurotrophin Signaling Pathway