CDK4 antibody (C-Term)

Catalog No. ABIN306814

This Rabbit Polyclonal antibody specifically detects CDK4 in WB and IP. It exhibits reactivity toward Human.

Quick Overview for CDK4 antibody (C-Term) (ABIN306814)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-CDK4 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Characteristics: p34cdk4 is a major catalytic subunit of mammalian D-type cyclins which is expressed by a gene found in human chromosome 12q13. The cdk4 protein is constitutively expressed throughout the cell cycle and it is the assembly and accumulation of functional cdk4/cyclin D complexes which is rate limiting for the progression through the G1 phase of the cell cycle. Once the complex is formed it must be activated by the phosphorylation of threonine 172 on the cdk4 subunit, a reaction mediated by a cdk-activating kinase (CAK). Complex formation and phosphorylation are independently regulated events. The phosphorylation of the cdk4 subunit is subject to regulation of both stimulatory and inhibitory cytokine signals, thereby allowing cdk4 to function as a mid G1 checkpoint. Independent of phosphorylation, cdk4 is also regulated by the binding of p16Ink4/mts1 while in complex with cyclin D.

Purification: Ammonium Sulfate Precipitation

Immunogen: TrpE-fusion protein containing the C-terminal 164 amino acids of human Cdk4

Isotype: IgG

Application Details

Application Notes: Detects Cdk4 by Western blot in HeLa cell lysates. Can also be used for immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: cyclin dependent kinase 4

UniProt: P11802

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size