Caspase 7 antibody

Catalog No. ABIN306833

The Mouse Monoclonal anti-Caspase 7 antibody is suitable to detect Caspase 7 in samples from Human. It has been validated for WB.

Quick Overview for Caspase 7 antibody (ABIN306833)

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 7 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: MCH3-5

Product Details anti-Caspase 7 Antibody

Purpose: Mouse anti Human Caspase-7

Cross-Reactivity: Human

Characteristics: Caspases are key effectors of programmed cell death. Caspase-7 along with caspase 3 and 6 form the group of effector caspases that are responsible for the cleavage of multiple substrates including the cytokeratins, PARP, alpha fodrin, NuMA and others. Caspase-7 is a 303 amino acid protein with high similarity to caspase-3. Caspase-7 occurs in three varient forms. Granzyme B activates pro-caspase-7 to a form which can cleave poly(ADP-ribose) polymerase (PARP) to its signature fragment of ~85 kDa. Also, in vivo caspase-7 appears to be a better substrate for granzyme B than caspase-3. Pro-caspase-7 has been shown to exist as dimers or high order oligomers. Caspase-7 may be an important intracellular effector of granzyme B-mediated apoptosis and cytotoxic T-lymophocyte-induced cell killing in vivo.

Purification: Ammonium Sulfate Precipitation

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human caspase-7 protein and mouse myeloma cells.

Isotype: IgG

Application Details

Application Notes: Detects proenzyme form of human Caspase-7 by Western blot. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Caspase 7

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Alternative Name: Caspase-7

Background: Cysteine-requiring Aspartate Protease-7, Apoptotic protease Mch-3, ICE-like apoptotic protease 3

UniProt: P55210

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity