HNRNPK antibody (C-Term)

Catalog No. ABIN307124

The Mouse Monoclonal anti-HNRNPK antibody is suitable to detect HNRNPK in samples from Human, Mouse, Rat, Rabbit and Chicken. It has been validated for WB and IHC.

Quick Overview for HNRNPK antibody (C-Term) (ABIN307124)

Target: HNRNPK (Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK))

Reactivity: Human, Mouse, Rat, Rabbit, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This HNRNPK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: F45 P9 C7

Product Details anti-HNRNPK Antibody

Binding Specificity: C-Term

Purpose: Mouse anti Human Heterogeneous Nuclear Ribonucleoprotein K

Cross-Reactivity: Chicken, Human, Mouse, Rabbit, Rat

Characteristics: Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA Polymerase II. There are approximately 20 known hnRNP proteins, which range in size from 34 kDa to 120 kDa, and their complexes are the major constituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus, however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K, a 65 kDa protein, recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminal of the hnRNP-K protein and mouse myeloma Ag8563 cells. Sequence common in human, mouse, rat, rabbit and chicken

Isotype: IgG1

Application Details

Application Notes: Antibody can be used for Western blotting (1-2 μg/mL) and immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-5 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for HNRNPK

Target: HNRNPK (Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK))

Alternative Name: Heterogeneous Nuclear Ribonucleoprotein K

Background: HnRNP-K, CSBP, TUNP, dC-Stretch Binding Protein

UniProt: P61978