SRPR antibody (AA 39-295)

Catalog No. ABIN307162

Product Details anti-SRPR Antibody

Target: SRPR (Signal Recognition Particle Receptor (Docking Protein) (SRPR))

Binding Specificity: AA 39-295

Reactivity: Dog

Host: Sheep

Clonality: Polyclonal

Conjugate: This SRPR antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Dog

Characteristics: Signal Recognition Particle Receptor, α subunit,The receptor for Signal Recognition Particle (SRP) is the site on the endoplasmic reticulum that ribosomes translating secreted and integral membrane proteins are initially targeted to. Once the ribosome-SRP complex arrives at the SRP receptor the protein being translated is transferred to the translocation complex (Sec61) in the ER membrane. The SRP receptor is composed of two subunits SRalpha and SRbeta. The SRalpha subunit is a translocation GTPase peripherally bound to the endoplasmic reticulum by its interaction with SRbeta. SRalpha also binds to the GTPase of SRP (SRP54) and these two proteins appear to function as each others GTPase activating proteins (GAPs). Hydrolysis of GTP by SRalpha and SRP54 is thought to be involved in transfer of the nascent protein to the Sec61 complex in the ER. SRalpha has an apparent molecular weight of 72 kDa. SRbeta is a Type I transmembrane protein that spans the membrane once and contains Ras type GTPase domain. The function of the GTPase in SRbeta is unknown. The membrane spanning domain is at the amino-terminus of SRbeta. The GTPase domain encompasses three quarters of the protein and is carboxyl- of the transmembrane region. SRalpha binds to the GTPase domain of SRbeta. Heterodimerization of SRalpha and SRbeta masks the carboxyl-terminal epitope of SRbeta.

Purification: Ammonium Sulfate Precipitation

Immunogen: The antibody to SRalpha was raised against a recombinant protein corresponding to amino acids 39-295 which includes part of the amino terminal SRbeta binding region and the hinge region between it and the carboxyl-terminal GTPase domain.

Isotype: IgG

Application Details

Application Notes: Detects SRalpha by Western blot analysis at 1 to 5 μg/mL. Also can be used to immunoprecipitate both subunits of SRP receptors under non-denaturing conditions. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for SRPR

Target: SRPR (Signal Recognition Particle Receptor (Docking Protein) (SRPR))

Alternative Name: SR alpha (SRPR Products)

Synonyms: sralpha antibody, srp-alpha antibody, 1300011P19Rik antibody, D11Mgi27 antibody, DP antibody, Sralpha antibody, SRP receptor alpha subunit antibody, signal recognition particle receptor (docking protein) antibody, signal recognition particle receptor subunit alpha antibody, signal recognition particle receptor antibody, signal recognition particle receptor (Docking protein) antibody, signal recognition particle receptor ('docking protein') antibody, srpra antibody, ftsY antibody, Tb11.01.1650 antibody, STER_1399 antibody, CMU_011790 antibody, EUBELI_01057 antibody, MGYG_00278 antibody, Tsp_06333 antibody, Srpr antibody, Srpra antibody, SRPRA antibody

Pathways: ER-Nucleus Signaling