HES1 antibody (AA 24-35)

Catalog No. ABIN307170

Cited in 1 publication. The Rabbit Polyclonal anti-HES1 antibody (ABIN307170) specifically detects HES1 in WB, IHC, IF, IHC (p) and IHC (fro). The antibody is reactive with Human, Mouse, Rat, Cow, Rabbit, Zebrafish (Danio rerio), Horse, Monkey, Bat, Xenopus laevis and Hamster samples.

Quick Overview for HES1 antibody (AA 24-35) (ABIN307170)

Target: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Zebrafish (Danio rerio), Horse, Monkey, Bat, Xenopus laevis, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HES1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HES1 Antibody

Binding Specificity: AA 24-35

Specificity: Recognizes human HES-1 (Hairy family).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Horse, Rabbit, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish (100%) Newt, Smelt (91%) Chicken (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa24-35 (TPDKPKTASEH) OF HES1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Horse, Rabbit, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish (100%), Newt, Smelt (91%), Chicken (82%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF, IHC, IHC-Fr, IHC-P (5 μg/mL), WB (1:200 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: Human neural stem cells, Raji cell lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

References for anti-HES1 antibody (ABIN307170)

Moya, Umans, Maas, Pereira, Beets, Francis, Sents, Robertson, Mummery, Huylebroeck, Zwijsen: "Stalk cell phenotype depends on integration of Notch and Smad1/5 signaling cascades." in: Developmental cell, Vol. 22, Issue 3, pp. 501-14, (2012) (PubMed).

Target Details for HES1

Target: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Alternative Name: HES1 / HES-1

Background: Name/Gene ID: HES1

Synonyms: HES1, BHLHb39, HHL, HL, Hairy and enhancer of split 1, Hairy homolog, HRY, Hairy homolog (Drosophila), Transcription factor HES-1, Hairy-like protein, HES-1

Gene ID: 3280

UniProt: Q14469

Pathways: DNA Damage Repair