MAG antibody (C-Term)

Catalog No. ABIN307172

The Rabbit Polyclonal anti-MAG antibody has been validated for WB, IHC, IF, IP and IHC (p). It is suitable to detect MAG in samples from Human and Rat.

Quick Overview for MAG antibody (C-Term) (ABIN307172)

Target: MAG (Myelin Associated Glycoprotein (MAG))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAG Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Reacts with the shared C-terminal region of the small and large myelin-associated glycoprotein.

Purification: Immunoaffinity purified

Immunogen: Human S/L-MAG fusion protein with a C-terminal region insert.

Type of Immunogen: Fusion protein

Application Details

Application Notes: Approved: IF, IHC, IHC-P (5 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunoprecipitation: 1-3 μg/IP reaction. Immunofluorescence: 10-30 μg/ul. Positive control: Rat brain homogenates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MAG

Target: MAG (Myelin Associated Glycoprotein (MAG))

Alternative Name: MAG

Background: Name/Gene ID: MAG
Family: CEA

Synonyms: MAG, GMA, Myelin associated glycoprotein, S-MAG, SIGLEC4A, SIGLEC-4A, Myelin-associated glycoprotein

Gene ID: 4099

UniProt: P20916

Pathways: Neurotrophin Signaling Pathway