TARDBP antibody (Internal Region)

Catalog No. ABIN307182

The Rabbit Polyclonal anti-TARDBP antibody (ABIN307182) specifically detects TARDBP in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TARDBP antibody (Internal Region) (ABIN307182)

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TARDBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TARDBP Antibody

Binding Specificity: Internal Region

Specificity: At least two isoforms are known to exist for this protein, this TDP43 antibody only recongizes the longer of the two isoforms.

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TARDBP

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Alternative Name: TDP-43 / TARDBP

Background: Name/Gene ID: TARDBP

Synonyms: TARDBP, ALS10, TAR DNA-binding protein-43, TDP-43, TAR DNA binding protein, TAR DNA-binding protein 43, TDP43

Gene ID: 23435

UniProt: Q13148

Pathways: Positive Regulation of Peptide Hormone Secretion