DNMT1 antibody (Internal Region)

Catalog No. ABIN308397

This anti-DNMT1 antibody is a Goat Polyclonal antibody detecting DNMT1 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for DNMT1 antibody (Internal Region) (ABIN308397)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DNMT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-DNMT1 Antibody

Binding Specificity: Internal Region

Purpose: DNMT1

Sequence: RFESPPKTQP TEDN

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-RFESPPKTQPTEDN

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear staining in PCT. Recommended concentration: 3-6 μg/mL.

Western Blot: Approx 170 kDa band observed in lysates of cell line Jurkat (calculated MW of 183 kDa according to NP_001370.1). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse for this p

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNMT1

Background: DNMT1, DNA (cytosine-5-)-methyltransferase 1, CXXC9, DNMT, FLJ16293, MCMT, MGC104992, DNA methyltransferase 1

Gene ID: 1786, 13433, 84350

NCBI Accession: NP_001370

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection