SPHK1 antibody (Internal Region)

Catalog No. ABIN308402

The Goat Polyclonal anti-SPHK1 antibody has been validated for WB, ELISA and IP. It is suitable to detect SPHK1 in samples from Human.

Quick Overview for SPHK1 antibody (Internal Region) (ABIN308402)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Grade: Verified

Product Details anti-SPHK1 Antibody

Binding Specificity: Internal Region

Purpose: SPHK1

Sequence: DVDLESEKYR RLGE

Specificity: This antibody is expected to recognise all three reported isoforms (NP_068807.2, NP_892010.2, NP_001136073.1). Reported variants represent identical protein (NP_001136073.1, NP_001136074.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DVDLESEKYRRLGE, from the internal region of the protein sequence according to NP_068807.2, NP_892010.2, NP_001136073.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2). Recommended concentration: 2-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunoprecipitation: EB05266 has been used in nuclear lysates of HeLa cells and a band of ~45kDa band has been detected.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: SPHK1

Background: SPHK1, sphingosine kinase 1, SPHK, SK1, SKase

Gene ID: 8877, 20698, 170897

NCBI Accession: NP_068807, NP_892010, NP_001136073

Pathways: VEGF Signaling