CD4 antibody

Catalog No. ABIN317082

The Rat Monoclonal anti-CD4 antibody (Clone RM4-5) (ABIN317082) specifically detects CD4 in FACS and IHC (fro). The antibody is reactive with Mouse samples.

Quick Overview for CD4 antibody (ABIN317082)

Target: CD4

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RM4-5

Product Details anti-CD4 Antibody

Specificity: This antibody RM4-5 detects CD4, a 55 kDa protein also known as Ly-4 and L3T4. The RM4-5 antibody is useful for in vitro blocking of ligand binding, as well as in vitro CD4+ T cell depletions.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Characteristics: Synonyms: T4/Leu-3, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: BALB/c mouse thymocytes

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product containsSodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled bytrained staff only.

Expiry Date: 12 months

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene ID: 12504

UniProt: P06332

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events