CD14 antibody (PE)

Catalog No. ABIN317123

The Mouse Monoclonal anti-CD14 antibody is suitable to detect CD14 in samples from Mouse. It has been validated for FACS.

Quick Overview for CD14 antibody (PE) (ABIN317123)

Target: CD14

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: BiG53

Product Details anti-CD14 Antibody (PE)

Characteristics: Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

Purification: Purified IgG prepared by affinity chromatography on Protein G

Immunogen: Recombinant mouse CD14

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of neat antibody to label 1x10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water

Buffer: PBS pH 7.4 containing 0.09 % Sodium Azide and 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: CD14 is a 55kD a glycophosphoinositol-linked cell surface protein that also exists in a soluble form. Expression of CD14 is restricted to the myeloid lineage and is predominantly expressed on peripheral blood monocytes, weakly expressed on granulocytes and absent on myeloid progenitors. The CD14 Molecule acts as a pattern recognition receptor, which binds lipopolysaccharide (LPS) and other microbial products such as lipoteichoic acid, lipoproteins and peptidoglycan. Binding of LPS to CD14 mediates the innate immune response to LPS and requires the Toll-like receptor-4 (TLR4)/MD-2 complex for effective signalling.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

UniProt: P10810

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades