CSF1R antibody

Catalog No. ABIN317381

The Rat Monoclonal anti-CSF1R antibody has been validated for FACS and Func. It is suitable to detect CSF1R in samples from Mouse and Rat.

Quick Overview for CSF1R antibody (ABIN317381)

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Reactivity: Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This CSF1R antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: 604B5 2E11

Product Details anti-CSF1R Antibody

Specificity: This antibody recognizes the Murine CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms. This antibody has been shown to inhibit in vitro colony formation in response to M-CSF in both rats and mice.

Characteristics: Synonyms: CSF-1-R, Fms proto-oncogene, c-fms, CSF1R, FMS, Macrophage colony-stimulating factor 1receptor

Purification: Purified IgG prepared by ion exchange chromatography

Immunogen: RAW 264 cells. Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3-653myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Functional Assays. Flow Cytometry: Use 10 μL of 1/10-1/100 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, None, None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody undiluted at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CSF1R

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Alternative Name: CD115

Background: This protein tyrosine kinase transmembrane receptor is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a member of the CSF1/PDGF receptor family of tyrosine protein kinases and contains 5 immunoglobulin like C2 type domains. CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Mutations in this gene have been associated with a predisposition to myeloid malignancy.Synonyms: CSF-1-R, CSF1R, FMS, Fms proto-oncogene, Macrophage colony-stimulating factor 1 receptor, c-fms

Gene ID: 12978

UniProt: P09581

Pathways: RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization