This antibody recognizes the Murine CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms. This antibody has been shown to inhibit in vitro colony formation in response to M-CSF in both rats and mice.
Functional Assays. Flow Cytometry: Use 10 μL of 1/10-1/100 diluted antibody to label 10^6 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4, None, None
Preservative
Without preservative
Storage
-20 °C
Storage Comment
Store the antibody undiluted at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
This protein tyrosine kinase transmembrane receptor is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a member of the CSF1/PDGF receptor family of tyrosine protein kinases and contains 5 immunoglobulin like C2 type domains. CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Mutations in this gene have been associated with a predisposition to myeloid malignancy.Synonyms: CSF-1-R, CSF1R, FMS, Fms proto-oncogene, Macrophage colony-stimulating factor 1 receptor, c-fms