IL-33 antibody (AA 112-270)

Catalog No. ABIN317536

The Mouse Monoclonal anti-IL-33 antibody has been validated for WB and EIA. It is suitable to detect IL-33 in samples from Human.

Quick Overview for IL-33 antibody (AA 112-270) (ABIN317536)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-33 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 4-00E-009

Product Details anti-IL-33 Antibody

Binding Specificity: AA 112-270

Specificity: The antibody recognizes human IL-33. Other species not tested.

Characteristics: Synonyms: IL-33, IL1F11, IL-1F11, NFHEV, NF-HEV, C9orf26, Interleukin-1 family member 11, Nuclear factorfrom high endothelial venules

Purification: Protein-G affinity chromatography

Immunogen: Recombinant human IL-33 (112-270 aa) purified from E. coli

Isotype: IgG2b

Application Details

Application Notes: ELISA. Western blot (1: 500 - 1: 2,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: Interleukin-33 (IL33)

Background: Interleukin 33 (IL-33) is a 32 kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T-cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12 kDa and C-terminal fragment of 18 kDa by cleavage of caspase-1.Synonyms: C9orf26, IL-1F11, IL-33, IL1F11, Interleukin-1 family member 11, NF-HEV, NFHEV, Nuclear factor from high endothelial venules

Gene ID: 90865

UniProt: O95760

Pathways: Production of Molecular Mediator of Immune Response