Prothrombin antibody

Catalog No. ABIN317606

The Rabbit Polyclonal anti-Prothrombin antibody is suitable to detect Prothrombin in samples from Human, Rat and Mouse. It has been validated for WB, IF and IHC (p).

Quick Overview for Prothrombin antibody (ABIN317606)

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prothrombin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Prothrombin Antibody

Specificity: This antibody detects endogenous levels of Prothrombin protein.(region surrounding Gly724)

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western Blot: 1/500-1/1000. ELISA: 1/10000-1/20000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Prothrombin

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Alternative Name: Prothrombin (F2)

Background: Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which mediate a blood coagulation cascade that leads to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Proteolytic cleavage of Prothrombin (Factor II) at residue 44 leads to formation of Thrombin in the first step of the coagulation cascade. Thrombin cleaves bonds between Arg and Gly and activates Factors V, VII, VIII and XIII in complex with thrombomodulin and Protein C. Thrombin maintains vascular integrity during development and postnatal life and coordinates connective tissue proteins by stimulating fibroblast procollagen production.Synonyms: Coagulation factor II

Molecular Weight: approx. 70 kDa

Gene ID: 2147

NCBI Accession: NP_000497

UniProt: P00734

Pathways: Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling