PIM1 antibody

Catalog No. ABIN317785

This Rabbit Polyclonal antibody specifically detects PIM1 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PIM1 antibody (ABIN317785)

Target: PIM1 (Pim-1 Oncogene (PIM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIM1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PIM1 Antibody

Specificity: This antibody detects endogenous levels of Pim-1 protein.(region surrounding Ile302)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/5000approx. 1/40000. Western Blot: 1/500approx. 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PIM1

Target: PIM1 (Pim-1 Oncogene (PIM1))

Alternative Name: PIM1

Background: Pim-1 is a serine/threonine kinase that cooperates with c-Myc in lymphoid cell transformation. The expression of Pim-1 increases during the progression from early to late G1, remaining high at the G1/S boundary and G2 phases of the cell cycle. Pim-1 is regulated at both the transcriptional and translational level, and it has been shown to be induced by IL-2 stimulation. Pim-1 also plays a role in T cell differentiation, and it has been shown to stimulate c-Myc-mediated apoptosis upstream of caspase-3-like proteases.Synonyms: Pim-1, Proto-oncogene serine/threonine-protein kinase pim-1

Molecular Weight: approx. 50 kDa

Gene ID: 5292

NCBI Accession: NP_002639

UniProt: P11309

Pathways: Glycosaminoglycan Metabolic Process