TPX2
Reactivity: Human
WB
Host: Mouse
Monoclonal
1696CT464-66-9
unconjugated
Application Notes
ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1,0 mg/mL
Buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
TPX2 (targeting protein for Xklp2) is a microtubule-associated protein involved in targeting the motor protein Xklp2 to microtubules. Ran-GTP activates TPX2 for the chromatin-induced microbutule assembly during M phase. Aurora-A kinase associates with TPX2 at the spindle apparatus and may regulate TPX2 via phosphorylation during the spindle assembly. TPX2 appears to play a structural role in spindle formation. TPX2 activates Eg2 in a microtubuledependent manner by stimulating the phosphorylation and kinase activity of Eg2. TPX2 is inactivated by binding to importin α, a nuclear import factor. Finally, the suppression of TPX2 with RNA interference causes defects in microtubule organization during mitosis.Synonyms: C20orf1, C20orf2, DIL-2, Differentially expressed in cancerous and non-cancerous lung cells 2, HCA519, Hepatocellular carcinoma-associated antigen 519, Restricted expression proliferation-associated protein 100, Targeting protein for Xklp2