FZD2 antibody

Catalog No. ABIN317860

This Rabbit Polyclonal antibody specifically detects FZD2 in WB and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for FZD2 antibody (ABIN317860)

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-FZD2 Antibody

Specificity: This antibody detects endogenous levels of Frizzled-2 protein.(region surrounding Glu240)

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FZD2

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Alternative Name: FZD2 / Frizzled-2

Background: The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity. The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy-terminal Ser/Thr-XXX-Val motif. The proteins in this family function as receptors for Wnt and are generally coupled to G proteins. Frizzleds are members of the G protein-coupled receptor superfamily. Frizzled-2 is expressed in the fetal kidney and lung and in the adult ovary and colon. Frizzled-2 mediates the Wnt/cGMP/Ca2+ pathway. It binds Wnt proteins and signals by activating the release of stored calcium. Frizzled-2 expression is regulated by Angiotensin II. Activated frizzled-2 suppresses the activity of protein kinase G, and activates NFAT-dependent transcription, the phosphatidylinositol pathway and calcium sensitive enzymes.Synonyms: Fz-2, FzE2, hFz2

Molecular Weight: approx. 55 kDa

Gene ID: 2535

NCBI Accession: NP_001457

UniProt: Q14332

Pathways: WNT Signaling