CDK5 antibody

Catalog No. ABIN317893

This Rabbit Polyclonal antibody specifically detects CDK5 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CDK5 antibody (ABIN317893)

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-CDK5 Antibody

Specificity: Cdk5 antibody detects endogenous levels of Cdk5 protein.(region surrounding Gly11)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry on paraffin sections: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CDK5

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Alternative Name: CDK5

Background: Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-3, PITALRE and PITSLRE. Cdk5 is thought to be involved in the G1/S transition of the cell cycle and is highly expressed in mature neurons. Activity of Cdk5 increases significantly during neuronal differentiation. Cdk5 has been postulated to be a neurofilament or Tau protein kinase, based on its ability to phosphorylate these proteins in vitro.Synonyms: Cell division protein kinase 5, Cyclin-dependent kinase 5, PSSALRE, Serine/threonine-protein kinase PSSALRE, TPKII catalytic subunit, Tau protein kinase II catalytic subunit

Molecular Weight: approx. 33 kDa

Gene ID: 1020

NCBI Accession: NP_001157882

UniProt: Q00535

Pathways: Cell Division Cycle, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis