Androgen Receptor antibody (pSer650)

Catalog No. ABIN317960

Product Details anti-Androgen Receptor Antibody

Target: Androgen Receptor (AR)

Binding Specificity: pSer650

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Androgen Receptor antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: P-Androgen Receptor (pSer650) antibody detects endogenous levels of p-Androgen Receptor protein.

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Androgen Receptor

Target: Androgen Receptor (AR)

Alternative Name: Androgen Receptor (AR Products)

Background: Androgens exhibit a wide range of effects on the development, maintenance and regulation of male phenotype and reproductive physiology in males. The androgen receptor (AR) is a member of the steroid superfamily of liganddependent transcription factors. ARs bind active testosterone (T) and dihydrotestosterone (DHT). The rates of association and dissociation of T are about three times more rapid than those of DHT. This difference in binding kinetic may account for the different physiological effects of T and DHT. Androgen binding results in an at least six-fold increase in androgen receptor stability. Akt phosphorylates human AR at Serines 210 and 790. The synthetic androgen R1881 elevates phosphorylation of Serines 308 and 650 in vitro.Synonyms: DHTR, Dihydrotestosterone receptor, NR3C4, Nuclear receptor subfamily 3 group C member 4

Gene ID: 367

NCBI Accession: NP_000035

UniProt: P10275

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding