NF-kB p65 antibody (pSer529)

Catalog No. ABIN3182088

The Rabbit Polyclonal anti-NF-kB p65 antibody has been validated for WB, IHC and ELISA. It is suitable to detect NF-kB p65 in samples from Human, Mouse, Rat and Monkey.

Quick Overview for NF-kB p65 antibody (pSer529) (ABIN3182088)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NF-kB p65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-NF-kB p65 Antibody

Binding Specificity: pSer529

Specificity: Phospho-NFκB-p65 (S529) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when phosphorylated at S529.

Characteristics: Rabbit Polyclonal to Phospho-NFκB-p65 (S529).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human NFkappaB-p65 around the phosphorylation site of S529.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:20000,

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: NFkappaB-p65

Molecular Weight: 60 kDa

Gene ID: 5970

UniProt: Q04206

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins