RAF1 antibody (pSer296)
The Rabbit Polyclonal anti-RAF1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect RAF1 in samples from Human, Mouse and Rat.
Quick Overview for RAF1 antibody (pSer296) (ABIN3182575)
Target
See all RAF1 Antibodies
RAF1
(V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
Reactivity
Human, Mouse, Rat
Host
Rabbit
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
Application
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
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Binding Specificity
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pSer296
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Specificity
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Phospho-Raf-1 (S296) Polyclonal Antibody detects endogenous levels of Raf-1 protein only when phosphorylated at S296.
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Characteristics
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Rabbit Polyclonal to Phospho-Raf-1 (S296).
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Immunogen
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Synthesized peptide derived from human Raf-1 around the phosphorylation site of S296.
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Isotype
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IgG
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Application Notes
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WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:20000,
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Restrictions
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For Research Use only
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Format
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Liquid
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Concentration
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1 mg/mL
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Buffer
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Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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Preservative
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Sodium azide
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Precaution of Use
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This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
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Avoid repeated freeze/thaw cycles.
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Storage
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-20 °C
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Storage Comment
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Store at -20°C.
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Target
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RAF1
(V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
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Alternative Name
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Raf-1
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Molecular Weight
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74 kDa
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Gene ID
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5894
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UniProt
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P04049
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Pathways
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MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
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