HA-Tag antibody

Catalog No. ABIN318728

The Rabbit Polyclonal anti-HA-Tag antibody is suitable to detect HA-Tag. It has been validated for WB, EIA and IHC (p).

Quick Overview for HA-Tag antibody (ABIN318728)

Target: HA-Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HA-Tag antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HA-Tag Antibody

Sequence: YPYDVPDYA

Purification: Purified

Immunogen: Keyhole limpet hemocyanin conjugated epitope tag peptide (114-122) from haemagglutinin influenza. A cysteine residue was used to facilitate coupling at the C-terminal end.

Isotype: IgG

Application Details

Application Notes: Immunohistochmemistry on paraffin sections (Requires antigen retrieval using heattreatment prior to staining of paraffin embedded sections. Citrate buffer is recommendedfor this purpose). ELISA. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.02 M Potassium phosphate, 0.12 M Sodium chloride pH 7.2, 0.01 % Sodium Azide (NaN3)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for HA-Tag

Target: HA-Tag

Alternative Name: HA Epitope Tag (YPYDVPDYA)

Target Type: Tag

Background: Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.