ASK1 antibody (pSer83)

Catalog No. ABIN318809

The Rabbit Polyclonal anti-ASK1 antibody is suitable to detect ASK1 in samples from Human. It has been validated for WB and EIA.

Quick Overview for ASK1 antibody (pSer83) (ABIN318809)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASK1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ASK1 Antibody

Binding Specificity: AA 76-87, pSer83

Specificity: This antibody detects Apoptosis Signal-regulating Kinase 1 (ASK1) phosphorylated at serine83. Akt in conjunction to Hsp90 phosphorylates ASK1 at Ser83 to inhibit ASK1 induced apoptosis.

Purification: Purified

Immunogen: KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 5,000 - 1: 10,000. Western Blot: 1: 1,000, detects a band of approximately 155 kDa in SW1353 cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.0mg/mL

Buffer: Phosphate buffered saline pH 7.2 containing 0.01 % Sodium Azide (NaN3)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Alternative Name: MEKK5 / ASK1

Background: ASK1 is a serine/threonine kinase also known as MAP kinase kinase kinase (MAPKKK), and plays an important role in the apoptotic response to various stimuli including oxidative stress, cytokines (e.g. TNF) and endoplasmic reticulum stress.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5

Gene ID: 4217

NCBI Accession: NP_005914

UniProt: Q99683

Pathways: MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response