Aurora Kinase B antibody (AA 227-238)

Catalog No. ABIN318827

The Rabbit Polyclonal anti-Aurora Kinase B antibody is suitable to detect Aurora Kinase B in samples from Human, Mouse, Rat, Monkey, Cow, Pig and Dog. It has been validated for WB and EIA.

Quick Overview for Aurora Kinase B antibody (AA 227-238) (ABIN318827)

Target: Aurora Kinase B (AURKB)

Reactivity: Human, Mouse, Rat, Monkey, Cow, Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora Kinase B antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Aurora Kinase B Antibody

Binding Specificity: AA 227-238

Specificity: This antibody detects Aurora-B kinase (AurB).

Cross-Reactivity (Details): Species reactivity (expected):Rat, Bovine, Mouse, Chimpanzee, Dog, Pig.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Synthetic peptide corresponding to amino acids 227-238 of human Aurora-B kinase.

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 2000 - 1: 10000. Western Blot: 1: 250 - 1: 1000, detects a band of approximately 39 kDa in HeLa cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: Phosphate buffered saline pH 7.2 containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: Aurora Kinase B

Background: Aurora-B kinase is a serine/threonine kinase which is the enzymatic core of the Chromosomal Passenger Complex (CPC). This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localisation, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis- one containing all four members, and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.Synonyms: AIK2, AIM1, ARK2, AURKB, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora/IPL1-related kinase 2, STK-1, STK12, Serine/threonine-protein kinase 12

Gene ID: 9212

NCBI Accession: NP_004208

UniProt: Q96GD4

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades