PPARA antibody (N-Term)

Catalog No. ABIN318836

The Rabbit Polyclonal anti-PPARA antibody has been validated for WB and EIA. It is suitable to detect PPARA in samples from Human.

Quick Overview for PPARA antibody (N-Term) (ABIN318836)

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PPARA Antibody

Binding Specificity: AA 1-18, N-Term

Specificity: This antibody detects an epitope within the N-terminal region of human peroxisome proliferator-activated receptor, alpha (PPARA).

Purification: Purified

Immunogen: Synthetic peptide corresponding to amino acids 1-18 of human PPAR alpha.

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 8000 - 1: 32000. Western Blot: 1: 500 - 1: 1000, detects a band of approximately 52 kDa in 3T3 whole celllysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0mg/mL

Buffer: Phosphate buffered saline pH 7.2 containing 0.09 % Sodium Azide (NaN3)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PPARA

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Alternative Name: PPAR-alpha

Background: PPARA is a member of the nuclear hormone receptor subfamily. Upon activation, PPARA forms heterodimers with retinoid X receptors (RXRs), and functions to regulate transcription of specific gene sequences. In particular, PPARA controls genes involved in the peroxisomal and mitochondrial beta oxidation pathways, fatty acid uptake, and triglyceride catabolism in the liver and skeletal muscle. PPARA also has anti-inflammatory and anti-proliferative effects and prevents the proatherogenic effects of cholesterol accumulation in macrophages by stimulating cholesterol efflux. PPARA is the main target of fibrate drugs, hypolipidemic agents used in the treatment of dyslipidemia and the prevention of cardiovascular disease. They could also be used for the treatment of type II diabetes.Synonyms: NR1C1, Nuclear receptor subfamily 1 group C member 1, PPAR, PPARA, Peroxisome proliferator-activated receptor alpha

Gene ID: 5465

NCBI Accession: NP_001001928

UniProt: Q07869

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C