ALOX5 antibody (pSer523)

Catalog No. ABIN318947

The Rabbit Polyclonal anti-ALOX5 antibody is suitable to detect ALOX5 in samples from Human, Rat and Monkey. It has been validated for WB.

Quick Overview for ALOX5 antibody (pSer523) (ABIN318947)

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Reactivity: Human, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ALOX5 Antibody

Binding Specificity: pSer523

Specificity: This antibody specifically recognises human 5-lipoxygenase (5-LO, also known as arachidonate 5-lipoxygenase) when phosphorylated at Ser523.

Purification: Purified

Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within human 5-lipoxygenase which includes phosphorylated Ser523.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1: 1000, detects a doublet of approximately 80 kDa in rat cortex lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES pH 7.5, 150 mM NaCl containing 0.09 % Sodium Azide, 0.01 % Bovine Serum Albumin, 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Alternative Name: ALOX5 / LOG5

Background: 5-LO is an enzyme that catalyses the synthesis of leukotrienes (LT's) from arachidonic acid (a polyunsaturated fatty acid), by the insertion of an oxygen into the arachidonic backbone. 5-LO requires calcium, ATP and the 5LOactivating protein as cofactors for full activity. Leukotrienes are synthesised and secreted by leukocytes in response to immunologic or inflammatory stimuli, such as in asthma and allergic reactions. The biological effects of LT's include leukocyte chemotaxis, pulmonary airway constriction, mucous hypersecretion, vasoconstriction and increased vascular permeability. I recent years 5-LO has also been implicated in the regulation of cell proliferation and apoptosis and has been found in a broad variety of cancer cells.Synonyms: 5-LO, 5-lipoxygenase, Arachidonate 5-lipoxygenase

Gene ID: 240

NCBI Accession: NP_000689

UniProt: P09917