Connexin 43/GJA1 antibody (pSer368)

Catalog No. ABIN319138

This Rabbit Polyclonal antibody specifically detects Connexin 43/GJA1 in WB. It exhibits reactivity toward Human, Rat, Mouse, Dog, Cow, Chicken, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for Connexin 43/GJA1 antibody (pSer368) (ABIN319138)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Human, Rat, Mouse, Dog, Cow, Chicken, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Connexin 43/GJA1 Antibody

Binding Specificity: pSer368

Specificity: This antibody specifically recognises 43 kDa Connexin43, when phosphorylated at Ser368.

Purification: Affinity chromatography

Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within connexin43 which includes phosphorylated Ser368

Isotype: IgG

Application Details

Application Notes: Western Blot: 1: 1000, detects a band of approximately 43 kDa in rat hippocampus celllysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES, pH 7.5 containing 0.09 % Sodium Azide, 0.01 % Bovine Serum Albumin, 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: GJA1 / Cx43

Background: Connexin43 (CX43) allows for the exchange of solutions between neighbouring cells through the formation of gap-junctional channels and is expressed in the brain, kidneys and myocardium, as well as vascular endothelial cells. Studies suggest that a decrease in the permeability of gap-junctional channels occurs due to a conformational change in the structure of CX43, and that this is mediated by the phosphorylation of CX43 at residue Ser368, by Protein Kinase C (PKC). A possible link between CX43 and epilepsy has been investigated following the detection of high levels of CX43 in both low-grade tumours and peritumoural reactive astrocytes (3).Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein

Gene ID: 2697

NCBI Accession: NP_000156

UniProt: P17302

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization