ENOS antibody (pSer1177)

Catalog No. ABIN319231

This Rabbit Polyclonal antibody specifically detects ENOS in IF and IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for ENOS antibody (pSer1177) (ABIN319231)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENOS antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ENOS Antibody

Binding Specificity: pSer1177

Specificity: Phospho-eNOS antibody (AP08011PU) detects endogenous levels of eNOS only when phosphorylated at serine 1177.

Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of Serine 1177 (T-Q-Sp-F-S).

Application Details

Application Notes: Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C.

Target Details for ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name: NOS3

Background: ENOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.Synonyms: Constitutive NOS, EC-NOS, Endothelial NOS, NOS type III, Nitric oxide synthase, eNOS, endothelial

Gene ID: 4846

NCBI Accession: NP_000594

UniProt: P29474

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling