HDAC2 antibody (pSer394)

Catalog No. ABIN319238

This Rabbit Polyclonal antibody specifically detects HDAC2 in WB, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HDAC2 antibody (pSer394) (ABIN319238)

Target: HDAC2 (Histone Deacetylase 2 (HDAC2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HDAC2 Antibody

Binding Specificity: pSer394

Specificity: This antibody HDAC2 (pSer394) detects endogenous levels of HDAC2 only when phosphorylated at Serine 394.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic phosphopeptide derived from human HDAC2 around the phosphorylation site of serine 394 (E-D-SP-G-D).

Application Details

Application Notes: Western blot (1/500-1/1000). Immunohistochemistry (1/50-1/100). Immunofluorescence (1/100-1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C.

Target Details for HDAC2

Target: HDAC2 (Histone Deacetylase 2 (HDAC2))

Alternative Name: HDAC2

Background: Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.Synonyms: HD2, Histone deacetylase 2

Gene ID: 3066

NCBI Accession: NP_001518

UniProt: Q92769

Pathways: Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection