CD4 antibody

Catalog No. ABIN319535

The Rat Monoclonal anti-CD4 antibody has been validated for FACS and IHC (fro). It is suitable to detect CD4 in samples from Mouse.

Quick Overview for CD4 antibody (ABIN319535)

Target: CD4

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: YTS191-1

Product Details anti-CD4 Antibody

Specificity: This antibody recognises the CD4 cell surface antigen, expressed by a subset of T-lymphocytes. This clone exhibits depleting activity when used in vivo (Antibody SM005LE is recommended for this purpose).

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, with 0.09 % sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene ID: 12504

NCBI Accession: NP_038516

UniProt: P06332

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events