PC antibody

Catalog No. ABIN3197484

There are 2+ publications for this product available. The Rabbit Polyclonal anti-PC antibody is suitable to detect PC in samples from Spinach, Zea mays, Synechocystis, Arabidopsis thaliana, Potato, Pea, Oryza sativa, Nicotiana tabacum, Mustard, Marchantia polymorpha, Lathyrus sativus, Helianthus annuus, Chlamydomonas reinhardtii and Barley. It has been validated for WB and EM.

Quick Overview for PC antibody (ABIN3197484)

Target: PC

Reactivity: Spinach, Zea mays, Synechocystis, Arabidopsis thaliana, Potato, Pea, Oryza sativa, Nicotiana tabacum, Mustard, Marchantia polymorpha, Lathyrus sativus, Helianthus annuus, Chlamydomonas reinhardtii, Barley

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Electron Microscopy (EM)

Product Details

Purpose: Anti-PC | Plastocyanin

Cross-Reactivity (Details): Arabidops thaliana, Brassica juncea, Chlamydomonas reinhardtii, Heliantus annuus, Hordeum vulgare, Lathyrus sativus, Marchantia polymorpha, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea, Solanum tuberosum, Synechocystis sp. PCC6803, Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Dicots,Chlamydomonas reinhardtii,Nicotiana benthamiana,Physcomitrium patens,Ricinus communis,Solanum lycopersicum,Suaeda salsa

Purification: Serum

Immunogen: Purified native plastocyanin from Spinacia oleracea UniProt: P00289

Application Details

Application Notes: 1 : 100 (IG), 1 : 2000 (WB)

Comment:

plastocyanin runs abberant due to negative charge at 12-19 kDa on SDS-PAGE depending upon the system used. in 15 % gel the protein will run closer to its true MW than in 12 % gel. In some cases PC can be very acidic and run at twice of its MW.PC1 runs closer to 14 kDa while PC2 runs closer to 19 kDa. For good resolution adding fresh DTT to the sample buffer is recommended. PC2 is generally more abundant and it increases with Cu feeding. PC1 is expressed first after etiolated seedlings are placed in the light.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Perea-García, Garcia-Molina, Andrés-Colás, Vera-Sirera, Pérez-Amador, Puig, Peñarrubia: "Arabidopsis copper transport protein COPT2 participates in the cross talk between iron deficiency responses and low-phosphate signaling." in: Plant physiology, Vol. 162, Issue 1, pp. 180-94, (2013) (PubMed).

Sikorskaite, Rajamäki, Baniulis, Stanys, Valkonen: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).

Target Details

Target: PC

Background:

Background: Plastocyanin (PC) is a small Cu protein and a mobile electron carrier in the lumen of the thylkoids. PC interacts with the B/F complex and Photosystem I. Alternative name: DNA-damage-repair/toleration protein DRT112.

Additional information: Cellular [compartment marker] of chloroplast thylakoid lumenThis product can be sold containing ProClin if requested.

Molecular Weight: 10 kDa

Gene ID: 838622

UniProt: P00289