ATP6V0D2 antibody (epsilon subunit)

Catalog No. ABIN3197488

There are 3+ publications for this product available. The Rabbit Polyclonal anti-ATP6V0D2 antibody is suitable to detect ATP6V0D2 in samples from Tomato, Nicotiana tabacum, Arabidopsis thaliana, Oryza sativa, Alfalfa, Zea mays, Vitis vinifera, Triticum aestivum, Thellungiella, Salicornia bigelovii, Pteris vittata (Chinese ladder brake), Poplar (Populus), Pineapple, Petunia hybrida, Noccaea caerulescens, Mesembryanthemum crystallinum, Lilium longiflorum (Trumpet lily), Fortunella margarita, Cucumis sativus, Chlamydomonas reinhardtii, Chara australis, Barley and Apple. It has been validated for WB, IF and IHC.

Quick Overview for ATP6V0D2 antibody (epsilon subunit) (ABIN3197488)

Target: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Reactivity: Tomato, Nicotiana tabacum, Arabidopsis thaliana, Oryza sativa, Alfalfa, Zea mays, Vitis vinifera, Triticum aestivum, Thellungiella, Salicornia bigelovii, Pteris vittata (Chinese ladder brake), Poplar (Populus), Pineapple, Petunia hybrida, Noccaea caerulescens, Mesembryanthemum crystallinum, Lilium longiflorum (Trumpet lily), Fortunella margarita , Cucumis sativus, Chlamydomonas reinhardtii, Chara australis, Barley, Apple

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V0D2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-ATP6V0D2 Antibody

Binding Specificity: epsilon subunit

Purpose: Anti-V-ATPase | Epsilon subunit of tonoplast H+ATPase

Cross-Reactivity (Details): Ananas comosus, Arabidopsis thaliana, Cucumis sativus, Chara australis , Chlamydomonas reinhardtii, Fortunella margarita Swingle, Hordeum vulgare, Lycopersicum esculentum, Lilium longiflorum, Malus x domestica Borkh. c.v. Fuji, Medicago truncatula, Mesembryanthemum crystallinum, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida cv. Mitchell, Populus sp., Pteris vittata (fern), Salicornia bigelovii, Thellungiella sp., Triticum aestivum, Zea mays, Vitis vinifera
Not reactive in: Avicennia sp., mangrove plants, Schizosaccharomyces pombe

Predicted Reactivity: Brachypodium dystachyon,Capsella rubella,Chenopodium quinoa,Citrus clementina,Citrus unshiu,Citrus limon,Eucalypsus grandis,Glyxine max,Glycine soja,Lotus japonicus,Phaseolus sp.,Physcomitrium patens,Populus trichocarpa,Prunus persica,Ricinus communis,Riticum aestivum,Solanum lycopersicum,Solanum tuberosum,Sorghum bicolor Theobroma cacao,Vitis vinifera,Bull frog,Chicken,Bovine,Drosophila melanogaster,Human,Mouse,Rat

Purification: Serum

Immunogen: KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana UniProt: Q39258-1

Application Details

Application Notes: 1: 100 (IF), 1 : 50 (IHC), 1 : 2000-1 : 5000 (WB)

Comment:

V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.Immunostaining protocol using V-ATPase antibodies can be found here.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-ATP6V0D2 antibody (ABIN3197488)

Bigeard, Rayapuram, Bonhomme, Hirt, Pflieger: "Proteomic and phosphoproteomic analyses of chromatin-associated proteins from Arabidopsis thaliana." in: Proteomics, Vol. 14, Issue 19, pp. 2141-55, (2014) (PubMed).

Schnell, Han, Miki, Johnson: "Soybean peroxidase propeptides are functional signal peptides and increase the yield of a foreign protein." in: Plant cell reports, (2010) (PubMed).

Hinton, Sennoune, Bond, Fang, Reuveni, Sahagian, Jay, Martinez-Zaguilan, Forgac: "Function of a subunit isoforms of the V-ATPase in pH homeostasis and in vitro invasion of MDA-MB231 human breast cancer cells." in: The Journal of biological chemistry, Vol. 284, Issue 24, pp. 16400-8, (2009) (PubMed).

Target Details for ATP6V0D2

Target: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Alternative Name: V-ATPase

Background:

Background: Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448

Additional information: Cellular [compartment marker] of tonoplast membrane.This product can be sold containing ProClin if required.

Molecular Weight: 26 | 31 kDa (Arabidopsis thaliana)

Gene ID: 826716

UniProt: Q39258

Pathways: Transition Metal Ion Homeostasis, Proton Transport