AGO4 antibody

Catalog No. ABIN3197495

The Rabbit Polyclonal anti-AGO4 antibody is suitable to detect AGO4 in samples from Arabidopsis thaliana and Hyacinthus orientalis (Common hyacinth). It has been validated for WB, IP, ICC and Co-IP.

Quick Overview for AGO4 antibody (ABIN3197495)

Target: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Reactivity: Arabidopsis thaliana, Hyacinthus orientalis (Common hyacinth)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGO4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Co-Immunoprecipitation ( Co-IP)

Product Details anti-AGO4 Antibody

Purpose: Anti-AGO4 | Argonaute 4

Cross-Reactivity (Details): Arabidopsis thaliana, Hyacinthus orientalis
Not reactive in: Brassica oleracea, Fragaria x ananassa, Hordeum vulgare, Poplar sp.,Solanum lycopersicum, Vigna angularis, Zea mays

Predicted Reactivity: Arabidopsis thaliana

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana AGO4 sequence UniProt: Q9ZVD5

Application Details

Application Notes: 1 : 100 (ICC), 5 μg of antibody per 1 gram of a fresh tissue (IP),1 : 2000-1 : 5000 (WB)

Comment:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa.Note that the AGO4 antibody reacts with the NEB prestained protein marker.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tube.

Target Details for AGO4

Target: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Alternative Name: AGO4

Background: AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Molecular Weight: 103 kDa

Gene ID: 817246

UniProt: Q9ZVD5

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Cellular Glucan Metabolic Process