TLR4 antibody (FITC)

Catalog No. ABIN319977

This anti-TLR4 antibody is a Mouse Monoclonal antibody detecting TLR4 in FACS. Suitable for Human, Dog, Pig, Guinea Pig and Monkey.

Quick Overview for TLR4 antibody (FITC) (ABIN319977)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human, Dog, Pig, Guinea Pig, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: HTA125

Product Details anti-TLR4 Antibody (FITC)

Purification: Affinity Chromatography on Protein G.

Immunogen: Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells or 100 μL whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: CD284 / TLR4

Background: TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 Molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.Synonyms: Toll-like receptor 4

NCBI Accession: NM_138554

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins