p53 antibody (acLys315)

Catalog No. ABIN3201007

The Mouse Monoclonal anti-p53 antibody has been validated for WB, ELISA, FACS and IP. It is suitable to detect p53 in samples from Human. There are 3+ publications available.

Quick Overview for p53 antibody (acLys315) (ABIN3201007)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 10E5

Product Details anti-p53 Antibody

Binding Specificity: acLys315

Characteristics: This product is the purified IgG fraction obtained from serum-free culture medium of mouse hybridoma (clone 10E5), which produces monoclonal antibody that specifically recognizes human p53 protein with acetylated Lys120.

Purification: Purified

Immunogen: synthetic peptide containing acLys315

Isotype: IgG1 kappa

Application Details

Application Notes: 1. Western blotting: ~1 g/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS ( pH 7.4), 50 % glycerol

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon arrival centrifuge briefly and store at -20 C.

References for anti-p53 antibody (ABIN3201007)

Tyteca, Legube, Trouche: "To die or not to die: a HAT trick." in: Molecular cell, Vol. 24, Issue 6, pp. 807-8, (2006) (PubMed).

Tang, Luo, Zhang, Gu: "Tip60-dependent acetylation of p53 modulates the decision between cell-cycle arrest and apoptosis." in: Molecular cell, Vol. 24, Issue 6, pp. 827-39, (2006) (PubMed).

Sykes, Mellert, Holbert, Li, Marmorstein, Lane, McMahon: "Acetylation of the p53 DNA-binding domain regulates apoptosis induction." in: Molecular cell, Vol. 24, Issue 6, pp. 841-51, (2006) (PubMed).

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53

Background: P53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells, the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Acetylation of lysine 120 (acetyl-K120) of p53 occurs rapidly after DNA damage and is catalyzed by the MYST family acetyltransferases hMOF and TIP60, and activates transcription of proapoptotic genes, BAX and PUMA.

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect