KDM5A antibody (AA 1416-1434)

Catalog No. ABIN3201015

There are 3+ publications for this product available. The Mouse Monoclonal anti-KDM5A antibody is suitable to detect KDM5A in samples from Human. It has been validated for WB, IF and FACS.

Quick Overview for KDM5A antibody (AA 1416-1434) (ABIN3201015)

Target: KDM5A (Lysine (K)-Specific Demethylase 5A (KDM5A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KDM5A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 18E8

Product Details anti-KDM5A Antibody

Binding Specificity: AA 1416-1434

Specificity: Specific to human and mouse RBP2. Can detect endogenous levels of RBP2.

Purification: Purified

Sterility: Sterile filtered

Immunogen: A synthetic peptide corresponding to human RBP2, aa 1416-1434.

Isotype: IgG2a kappa

Application Details

Application Notes: 1. Western blotting: ~1 g/mL
2. Immunofluorescence staining
3. Flow Cytometry: 1 g for 10^6 cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Preservative: Azide free

Storage: -20 °C

References for anti-KDM5A antibody (ABIN3201015)

Lopez-Bigas, Kisiel, Dewaal, Holmes, Volkert, Gupta, Love, Murray, Young, Benevolenskaya: "Genome-wide analysis of the H3K4 histone demethylase RBP2 reveals a transcriptional program controlling differentiation." in: Molecular cell, Vol. 31, Issue 4, pp. 520-30, (2008) (PubMed).

Christensen, Agger, Cloos, Pasini, Rose, Sennels, Rappsilber, Hansen, Salcini, Helin: "RBP2 belongs to a family of demethylases, specific for tri-and dimethylated lysine 4 on histone 3." in: Cell, Vol. 128, Issue 6, pp. 1063-76, (2007) (PubMed).

Klose, Yan, Tothova, Yamane, Erdjument-Bromage, Tempst, Gilliland, Zhang, Kaelin: "The retinoblastoma binding protein RBP2 is an H3K4 demethylase." in: Cell, Vol. 128, Issue 5, pp. 889-900, (2007) (PubMed).

Target Details for KDM5A

Target: KDM5A (Lysine (K)-Specific Demethylase 5A (KDM5A))

Alternative Name: KDM5A

Background: RBP2 was originally identified as a retinoblastoma binding protein. It is also known as JARID1A (Jumonji, AT rich interactive domain 1A). RBP2 plays both negative and positive roles in RB- mediated transcriptional activation, depending on the kinds of genes and regulates differentiation by its function as an H3K4 histone demethylase.

UniProt: P29375

Pathways: Chromatin Binding, Warburg Effect