IL-18 antibody (AA 37-193)

Catalog No. ABIN3201248

The Mouse Monoclonal anti-IL-18 antibody is suitable to detect IL-18 in samples from Human. It has been validated for WB and IHC.

Quick Overview for IL-18 antibody (AA 37-193) (ABIN3201248)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: C14

Product Details anti-IL-18 Antibody

Binding Specificity: AA 37-193

Purpose: Monoclonal Antibody to Interleukin 18 (IL18)

Sequence: MGHHHHHHSG S-YFGK LESKLSVIRN LNDQVLFIDQ GNRPLFEDMT DSDCRDNAPR TIFIISMYKD SQPRGMAVTI SVKCEKISTL SCENKIISFK EMNPPDNIKD TKSDIIFFQR SVPGHDNKMQ FESSSYEGYF LACEKERDLF KLILKKEDEL GDRSIMFTVQ NED

Specificity: The antibody is a mouse monoclonal antibody raised against IL18. It has been selected for its ability to recognize IL18 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Interleukin 18 (IL18) corresdonding to Tyr37~Asp193 with N-terminal His Tag

Isotype: IgG2a kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-40 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For long term storage aliquot into small volumes and store at-20°C in a manual defrost freezer for 24 months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: IL18

Background: IGIF, IL-1g, IL1F4, IL1-F4, Interferon-Gamma-Inducing Factor, Interleukin-1 Family Member 4, Iboctadekin, Interleukin-1 Gamma

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome