GLS2 antibody (AA 241-290)

Catalog No. ABIN320804

The Rabbit Polyclonal anti-GLS2 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect GLS2 in samples from Human, Mouse, Rat, Dog, Horse, Pig, Zebrafish (Danio rerio) and Bat.

Quick Overview for GLS2 antibody (AA 241-290) (ABIN320804)

Target: GLS2 (Glutaminase 2 (Liver, Mitochondrial) (GLS2))

Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Zebrafish (Danio rerio), Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLS2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GLS2 Antibody

Binding Specificity: AA 241-290

Specificity: Human GLS2 / Glutaminase 2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Horse (100%) Bovine, Rabbit, Guinea pig (92%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa241-290 of human GLS2 (Q9UI32). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bat, Horse, Pig, Opossum (100%), Marmoset, Bovine, Rabbit, Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 2.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.Antibody has been tested in IHC using formalin-fixed paraffin-embedded human tissues only.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GLS2

Target: GLS2 (Glutaminase 2 (Liver, Mitochondrial) (GLS2))

Alternative Name: GLS2 / Glutaminase 2

Background: Name/Gene ID: GLS2

Synonyms: GLS2, GA, Glutaminase 2, Glutaminase I, Glutaminase GA, L-glutaminase, HLGA, L-glutamine amidohydrolase, LGA, Breast cell glutaminase

Gene ID: 27165

UniProt: Q9UI32

Pathways: Dicarboxylic Acid Transport, Warburg Effect