SHC1 antibody (AA 371-420)

Catalog No. ABIN6738705

The Rabbit Polyclonal anti-SHC1 antibody (ABIN6738705) specifically detects SHC1 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Horse, Pig, Monkey, Guinea Pig, Rabbit, Xenopus laevis, Bat and Hamster samples.

Quick Overview for SHC1 antibody (AA 371-420) (ABIN6738705)

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Monkey, Guinea Pig, Rabbit, Xenopus laevis, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHC1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SHC1 Antibody

Binding Specificity: AA 371-420

Specificity: Human SHC1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Xenopus (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa371-420 of human SHC1 (P29353, NP_003020). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Xenopus (100%), Galago, Turkey, Chicken, Stickleback, Zebrafish (92%), Panda (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SHC1

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Alternative Name: p66 / SHC

Background: Name/Gene ID: SHC1

Synonyms: SHC1, p46 shc, p52 shc, SH2 domain protein C1, p66, SHCA, SHC, SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A

Gene ID: 6464

NCBI Accession: NP_003020

UniProt: P29353

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2