TGFB1 antibody (AA 151-200)

Catalog No. ABIN324431

The Rabbit Polyclonal anti-TGFB1 antibody is suitable to detect TGFB1 in samples from Human, Mouse, Pig, Cow, Sheep, Guinea Pig, Rabbit, Dog, Horse and Monkey. It has been validated for WB.

Quick Overview for TGFB1 antibody (AA 151-200) (ABIN324431)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivity: Human, Mouse, Pig, Cow, Sheep, Guinea Pig, Rabbit, Dog, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TGFB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TGFB1 Antibody

Binding Specificity: AA 151-200

Specificity: Mouse TGFB1 / TGF Beta

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Sheep, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Rat (92%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa151-200 of mouse Tgfb1 (P01137, NP_035707). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Ferret, Sheep, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Rat (92%), Bat (91%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: TGFB1 / TGF Beta 1

Background: Name/Gene ID: TGFB1
Family: TGF beta

Synonyms: TGFB1, CED, DPD1, Diaphyseal dysplasia 1, LAP, Latency-associated peptide, TGF Beta, TGFbeta, TGF-beta 1 protein, TGF-beta-1, TGFB

Gene ID: 7040

NCBI Accession: NP_035707

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints